Journal: NPJ Vaccines

Article Title: Parenteral vaccination protects against transcervical infection with Chlamydia trachomatis and generate tissue-resident T cells post-challenge

doi: 10.1038/s41541-020-0157-x

Figure Lengend Snippet: Groups of female B6C3F1 mice ( n = 8) were TC infected with different doses of C.t . SvD ranging from 10 3 to 10 6 IFU of C.t . SvD. 3 days and 7 days post infection, the bacterial burden in the uGT were determined in cultured swab samples. The data are presented as Log10 of IFUs. Bars indicate medians with IQR. Statistical significance was evaluated by a Kruskal-Wallis test followed by Dunn’s multiple comparisons test using GraphPad Prism version 7.04. (* p < 0.05, ** p < 0.01) ( a , b ). Percentages of neutrophils (Ly6G + CD11b + ), macrophages (Ly6G − CD11b + CD11c − ) and dendritic cells (Ly6G − CD11b + CD11c + ) were determined in the uGT and ILN 3 days post infection by flow cytometry. n = 8, animals were, pooled pairwise ( c , d ). Bars indicate means ± SD. Statistical significance was evaluated by an unpaired t-test using GraphPad Prism version 7.04. * p < 0.05, ** p < 0.01.

Article Snippet: Statistical significance was evaluated by an unpaired t-test using GraphPad Prism version 7.04.

Techniques: Infection, Cell Culture, Flow Cytometry

Journal: NPJ Vaccines

Article Title: Parenteral vaccination protects against transcervical infection with Chlamydia trachomatis and generate tissue-resident T cells post-challenge

doi: 10.1038/s41541-020-0157-x

Figure Lengend Snippet: Female B6C3F1 mice ( n = 8) were vaccinated three times s.c. with CTH522 antigen and CAF01 adjuvant, with two weeks intervals. Three weeks post immunization the mice received a TC infection with 10 3 IFU of C.t . SvD. The percentages of CD4 + T cells in uGT at day 3 post infection, was analyzed by flow cytometry (animals were pooled as 3 + 3 + 2). Bars indicate means ± SD. Statistical significance was evaluated by an unpaired t -test using GraphPad Prism version 7.04. **** p < 0.0001 ( a ). Three and 7 days post infection percentages of IL-17 negative CD4 T cells (Th1) and IL-17 positive CD4 cells (Th17) out of all CD4+ T cells in the uGT were analyzed by flow cytometry for the frequency of cytokine subsets (expression of TNFα, IL-2, IFNγ and/or IL-17). Gray bars indicate resting T cell subsets (expressing IL2 and/or TNFα), white bars indicate effector subsets that also express INFγ and black bars indicate subsets expressing only IFNγ within the Th1 and Th17 population ( b , c ). Representative contour plots of the cytokine expressions ( d ). Bacterial burdens in nonvaccinated, vaccinated and näive female B6C3F1 mice ( n = 16) were determined in cultured swab samples ( e ). Bars indicate medians with IQR. Statistical significance was evaluated by a Kruskal-Wallis test followed by Dunn’s multiple comparisons test using Graphpad Prism version 7.04. *** p < 0.001 ( e ).

Article Snippet: Statistical significance was evaluated by an unpaired t-test using GraphPad Prism version 7.04.

Techniques: Infection, Flow Cytometry, Expressing, Cell Culture